High risk HPV genotypes in Belarus

Results Of 764 PCR positive samples there were sequenced 77 and 10 DNAs on genes L1 (296 bp) and E6/E7 (620 bp) HPV-16 regions, respectively. The phylogenetic analysis of gene L1 region established that generally all samples from Belarus clustered round reference-sequences from South East Asia and Europe, only 15 and 9 samples formed independent groups with each other. p-distances between the Belarusian samples fluctuated from 0.000 to 0.024 that points as to new virus penetration to the country, and on circulation of our “old” HPV-16 types which formed independent groups of samples on a phylogenetic tree. Separately the sample 63 from Gomel settled down, it didn’t enter one of groups. Most likely, it is our “domestic” HPV-16 variant. Three viruses, two from Minsk and one from the Grodno region settled down in one group “Africa” with African reference sequences, but separately from each other that points to a different origin of a virus at these patients. Sequencing on gene of E6 and E6/E7 region also divided viruses into groups. Also as well as on L1 gene on gene E6/E7 region virus formed, generally the general groups with Asian HPV-16 variants. In too time part of isolates settled down separately and formed independent groups.